منابع مشابه
Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction.
We adapted a fusion polymerase chain reaction (PCR) strategy to synthesize gene disruption alleles of any sequenced yeast gene of interest. The first step of the construction is to amplify sequences flanking the reading frame we want to disrupt and to amplify the selectable marker sequence. Then we fuse the upstream fragment to the marker sequence by fusion PCR, isolate this product and fuse it...
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It was explained to the parents that there was a discrepancy between their child’s clinical profile and her investigation reports. In due course of time, the child revealed to the clinician and the parents that she had been applying her mother’s liquid vermillion to fake bleeding. Thus, she was diagnosed as a case of Munchausen Sydrome. She was referred for psychiatric treatment, which the pare...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.12.4895